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To link gene expression to metabolic changes, we performed an untargeted metabolomic analysis on H295R cells grown in the presence of 0 (control cells), 5 and 25 µM GSK126, through ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS)

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For amplification of transformation construct B (2476 bp, fusion of ptrA split marker with 3′-flanking region) primers oAoptrA.2 and oAfDUF323.6 were employed