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However, when present at supraphysiological concentrations, NO or RSNOs may induce nitrosative stress, mitochondrial dysfunction, and cell death 29,41,42
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Although CDO possessed appreciable catalytic activity in the absence of the Cys-Tyr cofactor, cofactor formation increased CDO catalytic efficiency by ~10-fold (e.g., 3,200 M −1 s −1 for wild-type CDO versus 220 M −1 s −1 for Cys93Ser mutant CDO, which lacks the Cys residue needed for formation of the Cys-Tyr cofactor)
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