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3T3-L1 cells were cultured in 60 mm diameter dishes (8.4×104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 μM dexamethasone, 10 μg/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)

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Additionally, tirzepatide has undergone cardiovascular outcomes trials, which assess its impact on cardiovascular events