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FIGURE 2 To distinguish which of these three sites are the dominant phosphorylation site of ArfGAP1, we generated recombinant GST-tagged ArfGAP1 proteins (GAP-F5 fragment) harboring individual phospho-null alanine mutations (S284A, T291A, and T292A) for in vitro radioactive kinase assays with recombinant GST-tagged ΔN-LRRK2 (970–2,527)

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Some investigators have suggested that the uremia of CKD is associated with decreased GH signalling contributing to the state of hepatic GH resistance (74) ( Figure 4 )