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Microsomal prostaglandin E synthase type 2 (mPGES2) is a glutathione-dependent heme protein, and dithiothreitol dissociates the bound heme to produce active prostaglandin E2 synthase in vitro
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Protein synthesis was assayed using the Click-iT Plus OPP Alexa Fluor 647 protein synthesis assay kit (Molecular Probes), according to the manufacturer’s instructions
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