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a total volume of 1 L CHO-3E7 cells + 1x antibiotic/antimycotics (GE Life Sciences, Marlborough, MA) was transfected with a total of 1 mg DNA (500 ⁇ g of antibody DNA and 500 ⁇ g of GFP/AKT/stuffer DNA) using PEI-MAX® (Polyscience, Inc., Philadelphia, PA) at a DNA:PEI ratio of 1:4 (w/w)
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BWA (version 0.5.9-r16) 54 with default parameters was used to perform genome-wide mapping of the adapter-trimmed reads to the yeast sacCer3 genome
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