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Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 2–3 × 10 5 cells per well in 500 µl complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 °C with 5% CO 2 for 16 h
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1,2 In the last several years, oral tofacitinib has become an increasingly utilized treatment option for immune-mediated skin conditions such as alopecia areata (AA), vitiligo, plaque psoriasis, and atopic dermatitis
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