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Figure 7 These data also raised the possibility that the ADA assay was not detecting true ADA, but 1:1 complexes of endogenous anti-TNFα antibodies and trimeric TNFα, since partially bound TNFα trimer could still theoretically bridge two molecules of AMG 966 in the antibody assay, and the etanercept spiked into the assay buffer to address this issue would not be able to neutralize partially bound TNFα

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Steinberg et al., 2019)