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To summarize, although very potent IRAP inhibitors have been discovered after systematic alterations starting from Ang IV none of the compounds are expected to reach the brain, at least not after oral administration

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Store all components at -20°C, and aliquot the biotinylated detection antibody and streptavidin-HRP conjugate into 50μL volumes to avoid repeated freeze-thaw cycles—these steps maintain antibody activity for up to 12 months