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Each Cy5-labelled DNA was added to hybridization buffer (20 µl DIG Easy Hybridization Buffer, 1 µl 10 mg/ml Yeast tRNA and 1 µl 10 mg/ml Salmon Sperm DNA) and hybridized for 18 hours at 50 °C followed by washing 3 × 1 × SSC/0.1% SDS at 42 °C and final wash in 0.1 × SSC at RT
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