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Insulin-like growth factor-1 deficiency and metabolic syndrome | Journal of Translational Medicine | Springer Nature Link

The primary substrates available for use by the working muscles are muscle and liver glycogen, blood glucose, intramuscular triglycerides and plasma free fatty acids (8, 33)

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The reaction was incubated for 1 h at room temperature prior to transfer onto GF/B filter plates (Perkin Elmer)