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3 Immunohistochemistry of interleukin-1β (IL-1β) in the dorsal root ganglion (DRG) and spinal cord (SC) at (A) 5 min (representative value of phase 1) and (B) 35 min (representative value of phase 2) Graph (bottom) represents the number of immunostained cells in the DRG and SC (mean ± SD)