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The residue was redissolved with acetonitrile-water (50:50, vol/vol) with 0.1% (vol/vol) formic acid, vortexed, and centrifuged at 14,000 rpm at 4°C for 15 min, and the supernatant was then injected into the liquid chromatography-tandem mass spectrometry (LC-MS/MS) system
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