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Extracellular acidification rates (ECAR) and real-time oxygen consumption rates (OCR) for MCF7 cells were determined using the Seahorse Extracellular Flux (XFe-96) analyzer (Seahorse Bioscience) [15]
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Continuous receptor activation isn't inherently superior to pulsed stimulation for compounds like Dihexa where the therapeutic effect derives from triggered structural changes rather than ongoing receptor occupancy
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