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The appropriate concentrations of DHLA and LA to set distinct redox potentials were calculated from the Nernst equation based on the standard reduction potential of lipoic acid ( E °′ LA ) of −290 mV at pH 7.0 (Lees and Whitesides, The redox potential for each of the two redox pairs is defined by the Nernst equation using the respective midpoint potential and ratios ( Q ) of oxidized to reduced roGFP and LA to DHLA, respectively (Equation 2)