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The reaction mixture was concentrated under reduced pressure, the residue was redissolved in EtOAc (20 mL), and the organic layer was washed with saturated aqueous NaHCO 3 (20 mL), brine (20 mL), dried over anhydrous Na 2 SO 4 , filtered, concentrated, and the crude product was purified by SiO 2 column chromatography (ISCO, 4 g) using 1–20% MeOH in DCM to get Boc-mitragynine as a white solid, (yield: 58 mg, 93%)